1. 建立逾2.5万家庭辅助生殖及自然妊娠出生队列

建立了我国最大的ART与自然妊娠人群同步纳入队列。截止目前，已完成超过3.5万个家庭的纳入工作，包括了2.2万个自然妊娠家庭和1.3万个辅助生殖妊娠家庭；累计采集调查问卷60余万份，生物样本100余万管。该队列以家庭为单位同时纳入辅助生殖妊娠和自然妊娠家庭，实行长期随访，积累了丰富的数据和生物样本资源，为我国开展高质量的出生队列研究奠定了坚实基础。

2. 建立完善的出生队列建设标准体系

为推进出生队列的标准化建设，项目组建立了涵盖研究对象纳入和随访、问卷调查、生物样本采集、质量控制等出生队列建设全流程的标准体系，并组织编写《出生队列建设标准与适宜技术》专著，制定《出生队列建设技术规范》系列标准（标准号：T/CPMA 015.1—2020；T/CPMA 015.2—2020；T/CPMA 015.3—2020）。这些成果对于提升我国出生队列建设的整体质量具有重要意义。

3. 建立高水平出生队列云端信息化平台

项目组开发了拥有自主知识产权的出生队列云端信息化平台系统，共获得软件著作权6项。基于该平台的现代化网络体系，项目组实现了对队列成员的实时化数据采集和高水平质量控制，使队列建设的整体质量得到显著提升，也为我国队列研究的信息化平台搭建提供了值得借鉴的经验。

4. 建成标准化出生队列专用生物样本实体库

样本库占地面积为1360平米，具有样本存储、样本处理、信息化管理和质量控制等功能，采用先进的生物样本管理系统对所有样本进行标记、定位、查询和出入库管理，同时采用综合监控平台对样本库的日常运行进行监控和管理。已建立一整套标准操作流程确保样本库的正常维护、运行和样本出入库质控。

5.聚焦重大科学问题，开展广泛的人群和基础研究

基于已建成的样本库和信息库，已获得多时点代谢组/蛋白质组暴露组学、 (金属元素/塑化剂/PFASs ）以及家系全基因组测序的多层次的组学数据，并进行初步分析。累计在Nature Medicine、Cell Research等高水平期刊发表研究论文50余篇。

[1] Wang C, Lv H, Ling X, Li H, Diao F, Dai J, Du J, Chen T, Xi Q, Zhao Y, Zhou K, Xu B, Han X, Liu X, Peng M, Chen C, Tao S, Huang L, Liu C, Wen M, Jiang Y, Jiang T, Lu C, Wu W, Wu D, Chen M, Lin Y, Guo X, Huo R, Liu J, Ma H, Jin G, Xia Y, Sha J, Shen H, Hu Z. Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study. Cell Res. 2021 Jun 9. doi: 10.1038/s41422-021-00521-w. Epub ahead of print. PMID: 34108666.

[2] Wang C, Gu Y, Zhou J, Zang J, Ling X, Li H, Hu L, Xu B, Zhang B, Qin N, Lv H, Duan W, Jiang Y, He Y, Jiang T, Chen C, Han X, Zhou K, Xu B, Liu X, Tao S, Jiang Y, Du J, Dai J, Diao F, Lu C, Guo X, Huo R, Liu J, Lin Y, Xia Y, Jin G, Ma H, Shen H, Hu Z. Leukocyte telomere length in children born following blastocyst-stage embryo transfer. Nat Med. 2022 Dec;28(12):2646-2653. doi: 10.1038/s41591-022-02108-3. Epub 2022 Dec 15. PMID: 36522605.

[3] Lv H, Diao F, Du J, Chen T, Meng Q, Ling X, Li H, Song C, Xi Q, Jiang Y, Xu Y, Tao S, Huang L, Wen M, Peng M, Liu C, Lu Q, He Y, Yin Y, Liu X, Xu B, Han X, Zhou K, Jiang T, Zhao Y, Ma H, Jin G, Xia Y, Liu J, Lin Y, Hu Z, Shen H. Assisted reproductive technology and birth defects in a Chinese birth cohort study. Lancet Reg Health West Pac. 2021 Jan 22;7:100090. doi: 10.1016/j.lanwpc.2020.100090. PMID: 34327418; PMCID: PMC8315325.

[4] Gu Y, Zhou Y, Ju S, Liu X, Zhang Z, Guo J, Gao J, Zang J, Sun H, Chen Q, Wang J, Xu J, Xu Y, Chen Y, Guo Y, Dai J, Ma H, Wang C, Jin G, Li C, Xia Y, Shen H, Yang Y, Guo X, Hu Z. Multi-omics profiling visualizes dynamics of cardiac development and functions. Cell Rep. 2022 Dec 27;41(13):111891. doi: 10.1016/j.celrep.2022.111891. PMID: 36577384.

[5] Lu Q, Lin Y, Chen T, Lv H, Diao F, Liu C, Peng M, Ling X, Li H, Wang Y, Wei Y, Du J, Jin G, Xia Y, Ma H, Liu X, Shen H, Hu Z. Alternations of gut microbiota composition in neonates conceived by assisted reproductive technology and its relation to infant growth. Gut Microbes. 2020 Nov 9;12(1):1794466. doi: 10.1080/19490976.2020.1794466. PMID: 32752913; PMCID: PMC7524295. 

[6] Du J, Lin Y, Xia Y, Ma H, Jiang Y, Lu C, Wu W, Chen M, Zhao Y, Dai J, Jin G, Liu J, Sha J, Shen H, Hu Z; China National Birth Cohort (CNBC) Study Group. Cohort Profile: The Jiangsu Birth Cohort. Int J Epidemiol. 2023 Nov 4:dyad139. doi: 10.1093/ije/dyad139. Epub ahead of print. PMID: 37935835.

[7] Lv H, Li X, Du J, Ling X, Diao F, Lu Q, Tao S, Huang L, Chen S, Han X, Zhou K, Xu B, Liu X, Ma H, Xia Y, Shen H, Hu Z, Jin G, Guan Y, Wang X. Effect of endometrial thickness and embryo quality on live-birth rate of fresh IVF/ICSI cycles: a retrospective cohort study. Reprod Biol Endocrinol. 2020 Aug 21;18(1):89. doi: 10.1186/s12958-020-00636-6. PMID: 32825835; PMCID: PMC7441697.

[8] Peng M, Wen M, Jiang T, Jiang Y, Lv H, Chen T, Ling X, Li H, Meng Q, Huang B, Tao S, Huang L, Liu C, Xu X, Lu Q, Liu X, Xu B, Han X, Zhou K, Chen J, Lin Y, Ma H, Xia Y, Shen H, Hu Z, Chen F, Du J, Jin G. Stress, anxiety, and depression in infertile couples are not associated with a first IVF or ICSI treatment outcome. BMC Pregnancy Childbirth. 2021 Oct 27;21(1):725. doi: 10.1186/s12884-021-04202-9. PMID: 34706683; PMCID: PMC8549180.

[9] Song C, Xu Y, Ding Y, Zhang Y, Liu N, Li L, Li Z, Du J, You H, Ma H, Jin G, Wang X, Shen H, Lin Y, Jiang X, Hu Z. The rates and medical necessity of cesarean delivery in China, 2012-2019: an inspiration from Jiangsu. BMC Med. 2021 Jan 25;19(1):14. doi: 10.1186/s12916-020-01890-6. PMID: 33487165; PMCID: PMC7831243.

[10] Lu S, Gu Y, Wu Y, Yang S, Li C, Meng L, Yuan W, Jiang T, Zhang X, Li Y, Wang C, Liu M, Ye L, Guo X, Shen H, Yang X, Tan Y, Hu Z. Bi-allelic variants in human WDR63 cause male infertility via abnormal inner dynein arms assembly. Cell Discov. 2021 Nov 16;7(1):110. doi: 10.1038/s41421-021-00327-5. PMID: 34782613; PMCID: PMC8593051.

[11] Wen M, Wu F, Du J, Lv H, Lu Q, Hu Z, Diao F, Ling X, Tan J, Jin G. Prediction of live birth probability after in vitro fertilization and intracytoplasmic sperm injection treatment: A multi-center retrospective study in Chinese population. J Obstet Gynaecol Res. 2021 Mar;47(3):1126-1133. doi: 10.1111/jog.14649. Epub 2021 Jan 4. PMID: 33398918.

[12] Li D, Yu C, Song C, Ning W, Xu Y, Ge H, Lin S, Zhou W, Lu Y, Wang X, Hu Z, Lin Y, Wu J. Maternal mortality ratio in Jiangsu Province, China: recent trends and associated factors. BMC Pregnancy Childbirth. 2021 Jun 25;21(1):447. doi: 10.1186/s12884-021-03897-0. PMID: 34172025; PMCID: PMC8235612.

[13] Du J, Li J, Liu X, Liu H, Obel C, Shen H, Hu Z, Yu Y. Association of maternal diabetes during pregnancy with high refractive error in offspring: a nationwide population-based cohort study. Diabetologia. 2021 Aug 17. doi: 10.1007/s00125-021-05526-z. Epub ahead of print. PMID: 34401952.

[14] Xu X, Tao S, Huang L, Du J, Liu C, Jiang Y, Jiang T, Lv H, Lu Q, Meng Q, Wang X, Qin R, Liu C, Ma H, Jin G, Xia Y, Kan H, Lin Y, Shen R, Hu Z; CNBC Study Group. Maternal PM2.5 exposure during gestation and offspring neurodevelopment: Findings from a prospective birth cohort study. Sci Total Environ. 2022 Oct 10;842:156778. doi: 10.1016/j.scitotenv.2022.156778. Epub 2022 Jun 18. PMID: 35724775.

[15] Lv S, Qin R, Jiang Y, Lv H, Lu Q, Tao S, Huang L, Liu C, Xu X, Wang Q, Li M, Li Z, Ding Y, Song C, Jiang T, Ma H, Jin G, Xia Y, Wang Z, Geng S, Du J, Lin Y, Hu Z. Association of Maternal Dietary Patterns during Gestation and Offspring Neurodevelopment. Nutrients. 2022 Feb 9;14(4):730. doi: 10.3390/nu14040730. PMID: 35215380; PMCID: PMC8878236.

[16] Wang Q, Jiang Y, Lv H, Lu Q, Tao S, Qin R, Huang L, Liu C, Xu X, Lv S, Li M, Li Z, Du J, Lin Y, Ma H, Chi X, Hu Z, Jiang T, Zhang G. Association of Maternal Mild Hypothyroidism With Offspring Neurodevelopment in TPOAb-Negative Women: A Prospective Cohort Study. Front Endocrinol (Lausanne). 2022 Jun 29;13:884851. doi: 10.3389/fendo.2022.884851. PMID: 35846339; PMCID: PMC9278520.

[17] Tao S, Du J, Chi X, Zhu Y, Wang X, Meng Q, Ling X, Diao F, Song C, Jiang Y, Lv H, Lu Q, Qin R, Huang L, Xu X, Liu C, Ding Y, Jiang T, Ma H, Xia Y, Liu J, Lin Y, Jin G, Hu Z; China National Birth Cohort (CNBC Study Group). Associations between antenatal corticosteroid exposure and neurodevelopment in infants. Am J Obstet Gynecol. 2022 Jun 3:S0002-9378(22)00435-5. doi: 10.1016/j.ajog.2022.05.060. Epub ahead of print. PMID: 35667419.

[18] Lin Y, Jiang Y, Du J, Ma H, Shen H, Hu Z. The continuing evolution of birth cohort studies: achievements and challenges†. Biol Reprod. 2022 Jul 25;107(1):358-367. doi: 10.1093/biolre/ioac117. PMID: 35686808.

[19] Dou Y, Yin Y, Li Z, Du J, Jiang Y, Jiang T, Guo W, Qin R, Li M, Lv H, Lu Q, Qiu Y, Lin Y, Jin G, Lu C, Ma H, Hu Z; China National Birth Cohort (CNBC Study Group). Maternal exposure to metal mixtures during early pregnancy and fetal growth in the Jiangsu Birth Cohort, China. Environ Res. 2022 Sep 10;215(Pt 2):114305. doi: 10.1016/j.envres.2022.114305. Epub ahead of print. PMID: 36096164.

[20] Qin R, Ding Y, Lu Q, Jiang Y, Du J, Song C, Lv H, Lv S, Tao S, Huang L, Xu X, Liu C, Jiang T, Wang Z, Ma H, Jin G, Xia Y, Hu Z, Zhang F, Lin Y. Associations of maternal dietary patterns during pregnancy and fetal intrauterine development. Front Nutr. 2022 Sep 15;9:985665. doi: 10.3389/fnut.2022.985665. PMID: 36185689; PMCID: PMC9520705.

[21] Yangqian Jiang, Yongyue Wei, Wenhui Guo, Jiangbo Du, Tao Jiang, Hongxia Ma, Guangfu Jin, Ting Chen, Rui Qin, Shiyao Tao, Qun Lu, Hong Lv, Xiumei Han, Kun Zhou, Bo Xu, Zhi Li, Mei Li, Yuan Lin, Yankai Xia, Zhibin Hu,Prenatal titanium exposure and child neurodevelopment at 1 year of age: A longitudinal prospective birth cohort study,Chemosphere,Volume 311, Part 1,2023,137034,ISSN 0045-6535,https://doi.org/10.1016/j.chemosphere.2022.137034.

[22] Wang Y, Qiu Y, Huang B, Du J, Liu L, Jiang T, Jiang Y, Lv H, Lu Q, Qin R, Tao S, Huang L, Xu X, Liu C, Ke K, Ma H, Shen H, Hu Z, Zhao Y, Lin Y; CNBC Study Group. Association between PM2.5 exposure and the outcomes of ART treatment: A prospective birth cohort study. Sci Total Environ. 2023 May 11;889:164099. doi: 10.1016/j.scitotenv.2023.164099. Epub ahead of print. PMID: 37178836.

[23] Wang Z, Dou Y, Guo W, Lin Y, Jiang Y, Jiang T, Qin R, Lv H, Lu Q, Jin G, Ma H, Hu Z, Liu H, Du J; China National Birth Cohort (CNBC Study Group). Association between prenatal exposure to trace elements mixture and visual acuity in infants: A prospective birth cohort study. Chemosphere. 2023 May 13;333:138905. doi: 10.1016/j.chemosphere.2023.138905. Epub ahead of print. PMID: 37187369.

[24] Huang B, Wang Y, Jiang Y, Lv H, Jiang T, Qiu Y, Lu Q, Du J, Lin Y, Ma H. Association of maternal hypertensive disorders in pregnancy with infant neurodevelopment. J Biomed Res. 2023 Sep 28:367-379. doi: 10.7555/JBR.37.20230074. Epub ahead of print. PMID: 37767602.

[25] Zang L, Lv H, Du J, Pan Y, Lin Y, Dai J. Association of phthalate exposure with low birth weight in couples conceiving naturally or via assisted reproductive technology in a prospective birth cohort. Sci Total Environ. 2023 Jan 10;855:158852. doi: 10.1016/j.scitotenv.2022.158852. Epub 2022 Sep 16. PMID: 36122707.

[26] 胡志斌.建设高质量出生队列支撑全生命周期人群健康研究[J].中华预防医学杂志,2018,52(10):973-975.

[27] 杜江波,陆群,靳光付,夏彦恺,沈洪兵,胡志斌.人群队列研究的数据管理与质量控制策略[J].中华预防医学杂志,2018,52(10):1078-1081.

[28] 沈洪兵. 大数据时代的临床医学研究——机遇和挑战[J]. 南京医科大学学报：自然科学版, 2020, 40(3):3.

[29] 胡志斌,杜江波,徐欣,林苑,马红霞,靳光付,李蓉,颜军昊,刘志伟,林戈,周灿权,夏彦恺,沈洪兵.中国国家出生队列建设背景和设计简介[J].中华流行病学杂志,2021,42(04):569-574.

[30] 蒋杨倩,胡志斌,杜江波,林苑,马红霞,靳光付,李蓉,颜军昊,刘志伟,林戈,周灿权,夏彦恺,沈洪兵.中国国家出生队列研究调查对象的基线特征分析[J].中华流行病学杂志,2021,42(04):579-585.

[31] 夏彦恺,蒋涛,刘聪,杜江波,林苑,蒋杨倩,赵杨,周昆,刘晓宇,靳光付,马红霞,胡志斌,沈洪兵.中国国家出生队列研究质量控制策略与措施[J].中华流行病学杂志,2021,42(04):575-578.

[32] 杜江波,陶诗瑶,林苑,赵杨,吕红,夏彦恺,陆春城,吴炜,马红霞,靳光付,胡志斌,沈洪兵.云端信息平台在中国国家出生队列建设与研究中的应用[J].中华流行病学杂志,2021,42(04):586-590

[33] 林苑,陆群,蒋杨倩,孟庆霞,王晓艳,刘聪,何元林,韩秀梅,周昆,杜江波,马红霞,靳光付,李红,凌秀凤,沈洪兵,胡志斌.中国国家出生队列母婴肠道微生物亚队列研究[J].中华流行病学杂志,2021,42(04):597-601.

[34] 杜江波,丁叶,黄磊,蒋杨倩,孟庆霞,宋词,吕红,刘晓宇,徐菠,林苑,马红霞,靳光付,李红,凌秀凤,柯晓燕,沈洪兵,胡志斌.中国国家出生队列孤独症谱系障碍亚队列建设概况[J].中华流行病学杂志,2021,42(04):591-596.

[35] 窦媛燕, 杜江波, 蒋杨倩, 宋词, 马红霞, 林苑, 沈洪兵, 胡志斌. 中国国家出生队列研究的理念与实践. 中华流行病学杂志, 2023, 44(1): 54-59